Multi-Omics Analysis of Anti-Inflammatory Action of Alkaline Extract of the Leaves of Sasa sp

Hiroshi Sakagami; Sachie Nakatani; Ayame Enomoto; Sana Ota; Miku Kaneko; Masahiro Sugimoto; Misaki Horiuchi; Kazuki Toeda; Takaaki Oizumi

doi:10.3390/jcm10102100

Multi-Omics Analysis of Anti-Inflammatory Action of Alkaline Extract of the Leaves of Sasa sp

J Clin Med. 2021 May 13;10(10):2100. doi: 10.3390/jcm10102100.

Authors

Hiroshi Sakagami¹, Sachie Nakatani², Ayame Enomoto³, Sana Ota³, Miku Kaneko³, Masahiro Sugimoto^{3

4}, Misaki Horiuchi⁵, Kazuki Toeda⁵, Takaaki Oizumi⁵

Affiliations

¹ Meikai University Research Institute of Odontology (M-RIO), Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan.
² Graduate School of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
³ Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, Japan.
⁴ Research and Development Center for Minimally Invasive Therapies, Institute of Medical Science, Tokyo Medical University, Shinjuku, Tokyo 160-0022, Japan.
⁵ Daiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.

Abstract

Efficient utilization of alkaline extracts of several plants for the treatment of oral diseases has been reported. To investigate the mechanism of anti-inflammatory activity of alkaline extract of the leaves of Sasa sp. (SE), multi-omics analysis using metabolomics and DNA array was performed. Human gingival fibroblasts (HGFs) were treated for IL-1β to induce inflammation (detected by PGE₂ production in culture medium) in the presence or absence of SE. Both IL-1β and SE showed slight hormetic growth stimulation against HGF. SE inhibited PGE₂ production dose- and time-dependently. Its inhibitory action was more pronounced by first treating the cells with SE, rather than with IL-1β. At 3 h after IL-1β treatment, 18 amino acids (except cysteine and glutamic acid), total glutathione (GSH, GSSG, Cys-GSH disulfide), Met-sulfoxide, 5-oxoproline, and SAM declined, whereas DNA expressions of AKT, CASP3, and CXCL3 were elevated. These changes were reversed by simultaneous treatment with SE. The present study suggests that the anti-inflammatory action of SE is mediated via various metabolic pathways for cell survival, apoptosis, and leukocyte recruitment.

Keywords: DNA array; IL-1β; PGE2; alkaline extract of the leaves of Sasa sp.; anti-inflammation; apoptosis; cell survival; human gingival fibroblast; metabolomics.