Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis

Stem Cell Res. 2021 May:53:102370. doi: 10.1016/j.scr.2021.102370. Epub 2021 Apr 27.

Abstract

Multiple sclerosis is a chronic disease characterized by inflammation, demyelination, and axonal damage in the central nervous system. Here, we established an induced pluripotent stem cell (iPSC) line METUi001-A from the peripheral blood mononuclear cells of a 25-year-old male individual with clinically diagnosed Relapsing-Remitting Multiple Sclerosis (RRMS) using the integration-free Sendai reprogramming method. We demonstrated that the iPSCs are free of exogenous Sendai reprogramming vectors, have a normal male karyotype, express pluripotency markers, and differentiate into the three germ layers. The iPSC line can serve as a valuable resource to generate cellular model systems to investigate molecular mechanisms underlying RRMS.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • Humans
  • Induced Pluripotent Stem Cells*
  • Karyotype
  • Leukocytes, Mononuclear
  • Male
  • Multiple Sclerosis*
  • Multiple Sclerosis, Relapsing-Remitting*
  • Sendai virus / genetics