Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine

Methods Mol Biol. 2021:2275:291-299. doi: 10.1007/978-1-0716-1262-0_18.

Abstract

Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy.

Keywords: Fluorescence imaging; MitoSOX Red®; Mitochondrial membrane potential.

MeSH terms

  • Cells, Cultured
  • Dicarbethoxydihydrocollidine / analogs & derivatives*
  • Dicarbethoxydihydrocollidine / chemistry
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fluorescent Dyes / chemistry*
  • HEK293 Cells
  • Humans
  • Membrane Potential, Mitochondrial
  • Microscopy, Fluorescence
  • Mitochondria / metabolism*
  • Myoblasts / cytology
  • Reactive Oxygen Species / analysis*
  • Skin / cytology
  • Skin / metabolism

Substances

  • Fluorescent Dyes
  • Reactive Oxygen Species
  • dihydroethidine
  • Dicarbethoxydihydrocollidine