Alterations in bile acid metabolizing gut microbiota and specific bile acid genes as a precision medicine to subclassify NAFLD

Physiol Genomics. 2021 Aug 1;53(8):336-348. doi: 10.1152/physiolgenomics.00011.2021. Epub 2021 Jun 21.

Abstract

Multiple mechanisms for the gut microbiome contributing to the pathogenesis of nonalcoholic fatty liver disease (NAFLD) have been implicated. Here, we aim to investigate the contribution and potential application for altered bile acids (BA) metabolizing microbes in NAFLD by post hoc analysis of whole metagenome sequencing (WMS) data. The discovery cohort consisted of 86 well-characterized patients with biopsy-proven NAFLD and 38 healthy controls. Assembly-based analysis was performed to identify BA-metabolizing microbes. Statistical tests, feature selection, and microbial coabundance analysis were integrated to identify microbial alterations and markers in NAFLD. An independent validation cohort was subjected to similar analyses. NAFLD microbiota exhibited decreased diversity and microbial associations. We established a classifier model with 53 differential species exhibiting a robust diagnostic accuracy [area under the receiver-operator curve (AUC) = 0.97] for detecting NAFLD. Next, eight important differential pathway markers including secondary BA biosynthesis were identified. Specifically, increased abundance of 7α-hydroxysteroid dehydrogenase (7α-HSDH), 3α-hydroxysteroid dehydrogenase (baiA), and bile acid-coenzyme A ligase (baiB) was detected in NAFLD. Furthermore, 10 of 50 BA-metabolizing metagenome-assembled genomes (MAGs) from Bacteroides ovatus and Eubacterium biforme were dominant in NAFLD and interplayed as a synergetic ecological guild. Importantly, two subtypes of patients with NAFLD were observed according to secondary BA metabolism potentials. Elevated capability for secondary BA biosynthesis was also observed in the validation cohort. These bacterial BA-metabolizing genes and microbes identified in this study may serve as disease markers. Microbial differences in BA-metabolism and strain-specific differences among patients highlight the potential for precision medicine in NAFLD treatment.

Keywords: NAFLD; gut microbiota; secondary BA synthesis; whole metagenome sequencing data.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) / genetics
  • 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) / metabolism
  • Bile Acids and Salts / genetics*
  • Bile Acids and Salts / metabolism*
  • Case-Control Studies
  • Coenzyme A Ligases / genetics
  • Coenzyme A Ligases / metabolism
  • Female
  • Gastrointestinal Microbiome* / genetics
  • Gastrointestinal Microbiome* / physiology
  • Humans
  • Hydroxysteroid Dehydrogenases / genetics
  • Hydroxysteroid Dehydrogenases / metabolism
  • Male
  • Middle Aged
  • Non-alcoholic Fatty Liver Disease / genetics*
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Non-alcoholic Fatty Liver Disease / microbiology*
  • Precision Medicine
  • Reproducibility of Results

Substances

  • Bile Acids and Salts
  • Hydroxysteroid Dehydrogenases
  • 7 alpha-hydroxysteroid dehydrogenase
  • 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
  • Coenzyme A Ligases
  • bile acid-CoA ligase