P1 gene of Mycoplasma pneumoniae isolated from 2016 to 2019 and relationship between genotyping and macrolide resistance in Hokkaido, Japan

Nobuhisa Ishiguro; Rikako Sato; Hideaki Kikuta; Masanori Nakanishi; Hayato Aoyagi; Toshihiko Mori; Naoko Nagano; Yuichi Tabata; Kyosuke Hazama; Mutsuko Konno; Tatsuru Yamanaka; Katsumi Azuma; Hiroshi Tanaka; Mitsuo Narita; Keisuke Morita; Yasuhisa Odagawa; Akihito Ishizaka; Akira Tsuchida; Satoshi Sasaki; Atsuko Horino; Tsuyoshi Kenri; Takehiro Togashi; Atsushi Manabe

doi:10.1099/jmm.0.001365

P1 gene of Mycoplasma pneumoniae isolated from 2016 to 2019 and relationship between genotyping and macrolide resistance in Hokkaido, Japan

J Med Microbiol. 2021 Jun;70(6). doi: 10.1099/jmm.0.001365.

Authors

Nobuhisa Ishiguro¹, Rikako Sato¹, Hideaki Kikuta², Masanori Nakanishi³, Hayato Aoyagi⁴, Toshihiko Mori⁵, Naoko Nagano⁶, Yuichi Tabata⁷, Kyosuke Hazama⁸, Mutsuko Konno⁹, Tatsuru Yamanaka¹⁰, Katsumi Azuma¹¹, Hiroshi Tanaka¹², Mitsuo Narita¹³, Keisuke Morita¹⁴, Yasuhisa Odagawa¹⁵, Akihito Ishizaka¹⁶, Akira Tsuchida¹⁷, Satoshi Sasaki¹⁸, Atsuko Horino¹⁹, Tsuyoshi Kenri¹⁹, Takehiro Togashi²⁰, Atsushi Manabe¹

Affiliations

¹ Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan.
² Pediatric Clinic, Touei Hospital, Sapporo, Hokkaido, Japan.
³ Department of Pediatrics, Kushiro Red Cross Hospital, Kushiro, Hokkaido, Japan.
⁴ Department of Pediatrics, Obihiro Kyokai Hospital, Obihiro, Hokkaido, Japan.
⁵ Department of Pediatrics, NTT East Sapporo Hospital, Sapporo, Hokkaido, Japan.
⁶ Nagano Pediatric Clinic, Asahikawa, Hokkaido, Japan.
⁷ Iwamizawa Pediatric and Gynecology Clinic, Iwamizawa, Hokkaido, Japan.
⁸ Hazama Pediatric Clinic, Muroran, Hokkaido, Japan.
⁹ Department of Pediatrics, Sapporo Kosei General Hospital, Sapporo, Hokkaido, Japan.
¹⁰ Yamanaka Tatsuru Pediatric Clinic, Sapporo, Hokkaido, Japan.
¹¹ Azuma Kodomo-Katei Clinic, Ebetsu, Hokkaido, Japan.
¹² Sapporo Cough Asthma Allergy Center, Sapporo, Hokkaido, Japan.
¹³ Department of Pediatrics, Sapporo Tokushukai Hospital, Sapporo, Hokkaido, Japan.
¹⁴ Department of Pediatrics, Red Cross Asahikawa Hospital, Asahikawa, Hokkaido, Japan.
¹⁵ Department of Pediatircs, Otaru General Hospital, Otaru, Hokkaido, Japan.
¹⁶ Sumiyoshi Pediatric Clinic, Chitose, Hokkaido, Japan.
¹⁷ Tsuchida Pediatric Clinic, Asahikawa, Hokkaido, Japan.
¹⁸ Department of Pediatrics, Aiiku Hospital, Sapporo, Hokkaido, Japan.
¹⁹ Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
²⁰ Hokkaido Anti-Tuberculosis Association Sapporo Fukujuji Clinic, Sapporo, Hokkaido, Japan.

PMID: 34165424
DOI: 10.1099/jmm.0.001365

Abstract

We characterized 515 Mycoplasma pneumoniae specimens in Hokkaido. In 2013 and 2014, the p1 gene type 1 strain, mostly macrolide-resistant, was dominant and the prevalence of macrolide resistance was over 50 %. After 2017, the p1 gene type 2 lineage, mostly macrolide-sensitive, increased and the prevalence of macrolide resistance became 31.0 % in 2017, 5.3 % in 2018 and 16.3 % in 2019.

Keywords: Japan; Mycoplasma pneumoniae; antibiotic resistance; macrolides.

MeSH terms

Child
Drug Resistance, Bacterial / genetics
Genotyping Techniques / methods
Humans
Japan / epidemiology
Macrolides / pharmacology*
Mutation
Mycoplasma pneumoniae / classification
Mycoplasma pneumoniae / drug effects
Mycoplasma pneumoniae / genetics*
Nasopharynx / microbiology
Pneumonia, Mycoplasma / drug therapy
Pneumonia, Mycoplasma / epidemiology*
Pneumonia, Mycoplasma / microbiology
RNA, Ribosomal, 23S / genetics

Substances

Macrolides
RNA, Ribosomal, 23S