RNA-Sequencing Analysis of Differentially Expressed Genes in Human iPSC-Derived Cardiomyocytes

Methods Mol Biol. 2021:2320:193-217. doi: 10.1007/978-1-0716-1484-6_19.

Abstract

RNA sequencing profiles and characterizes cell and tissue samples, giving important insights into molecular mechanisms. Such data is imperative for cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) and used in related translational and basic research. Here we provide reliable protocols to extract differentially expressed genes in iPSC-CMs with RNA sequencing.

Keywords: Gene expression; Genome-wide analysis; Molecular biology; Next-generation sequencer; RNA sequencing; Transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / genetics
  • Cells, Cultured
  • Chromosome Mapping
  • Gene Expression Profiling
  • Gene Library
  • Genes, Reporter
  • Genome, Human
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • MicroRNAs / genetics
  • Myocytes, Cardiac / chemistry*
  • Principal Component Analysis
  • RNA / isolation & purification
  • RNA-Seq / methods*
  • Sequence Alignment
  • Software
  • Transcriptome*

Substances

  • MicroRNAs
  • RNA