First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection

Viruses. 2021 Jul 1;13(7):1290. doi: 10.3390/v13071290.

Abstract

Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV), referred to as small ruminant lentiviruses (SRLVs), belong to the genus Lentivirus of the Retroviridae family. SRLVs infect both sheep and goats, causing significant economic losses and animal welfare damage. Recent findings suggest an association between serological status and allelic variants of different genes such as TMEM154, TLR9, MYD88 and CCR5. The aim of this work was to investigate the role of specific polymorphisms of these genes in SRLVs infection in some sheep flocks in Italy. In addition to those already known, novel variants in the TMEM154 (P7H, I74V, I105V) gene were detected in this study. The risk of infection was determined finding an association between the serological status and polymorphisms P7H, E35K, N70I, I74V, I105V of TMEM154, R447Q, A462S and G520R in TLR9 gene, H176H* and K190K* in MYD88 genes, while no statistical association was observed for the 4-bp deletion of the CCR5 gene. Since no vaccines or treatments have been developed, a genetically based approach could be an innovative strategy to prevent and to control SRLVs infection. Our findings are an important starting point in order to define the genetic resistance profile towards SRLVs infection.

Keywords: chemokine (C-C motif) receptor 5 (CCR5); genetic resistance; myeloid differentiation primary response 88 (MYD88); relative risk; small ruminant lentiviruses (SRLVs); toll-like receptor 9 (TLR9); transmembrane protein 154 (TMEM154).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Resistance / genetics*
  • Genetic Variation
  • Italy
  • Lentivirus / classification
  • Lentivirus / genetics*
  • Lentivirus Infections / genetics*
  • Lentivirus Infections / immunology
  • Lentivirus Infections / prevention & control
  • Lentivirus Infections / veterinary*
  • Membrane Proteins / classification
  • Membrane Proteins / genetics*
  • Membrane Proteins / immunology
  • Myeloid Differentiation Factor 88 / genetics*
  • Polymorphism, Single Nucleotide*
  • Receptors, CCR5 / genetics*
  • Risk Factors
  • Sheep
  • Sheep Diseases / genetics
  • Sheep Diseases / immunology
  • Sheep Diseases / virology
  • Toll-Like Receptor 9 / genetics*

Substances

  • Membrane Proteins
  • Myeloid Differentiation Factor 88
  • Receptors, CCR5
  • Toll-Like Receptor 9