Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum

Yong Yang; Changjie Lv; Junqing Fan; Ya Zhao; Lili Jiang; Xiaomei Sun; Qiang Zhang; Meilin Jin

doi:10.1016/j.jviromet.2021.114269

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum

J Virol Methods. 2021 Dec:298:114269. doi: 10.1016/j.jviromet.2021.114269. Epub 2021 Aug 25.

Authors

Yong Yang¹, Changjie Lv¹, Junqing Fan¹, Ya Zhao², Lili Jiang¹, Xiaomei Sun¹, Qiang Zhang³, Meilin Jin⁴

Affiliations

¹ National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
² National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China; College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, 430070, China.
³ College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 05yisan@163.com.
⁴ National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, 430070, China. Electronic address: jml8328@126.com.

PMID: 34454001
DOI: 10.1016/j.jviromet.2021.114269

Abstract

The outbreak of African swine fever (ASF) has caused significant economic losses to animal husbandry worldwide. Currently, there is no effective vaccine or treatment available to control the disease, and therefore, efﬁcient disease control is dependent on early detection and diagnosis of ASF virus (ASFV). In this study, a chemiluminescent immunoassay (CLIA) was developed using the ASFV protein p54 as a serum diagnostic antigen and an anti-p54 monoclonal antibody. After optimizing the working parameters of the CLIA, the sensitivity of the established CLIA was 1:128, ASFV-specific serum antibody was identified, and there was no cross-reaction with other swine virus antibodies. After testing 49 clinical serum samples, the consistency rate between the CLIA and the World Organization for Animal Health (OIE) recommended commercial kit was 100 %. Thus, this CLIA had a high degree of specificity, sensitivity, and reliability, and could be used as a rapid detection method for epidemiological investigations of ASFV infection.

Keywords: African swine fever virus; Chemiluminescence immunoassay; Monoclonal antibody; p54 protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

African Swine Fever Virus*
African Swine Fever*
Animals
Immunoassay
Luminescence
Reproducibility of Results
Swine