Three Reasons Why Aspartic Acid and Glutamic Acid Sequences Have a Surprisingly Different Influence on Mineralization

J Phys Chem B. 2021 Sep 16;125(36):10335-10343. doi: 10.1021/acs.jpcb.1c04467. Epub 2021 Sep 2.

Abstract

Understanding the role of polymers rich in aspartic acid (Asp) and glutamic acid (Glu) is the key to gaining precise control over mineralization processes. Despite their chemical similarity, experiments revealed a surprisingly different influence of Asp and Glu sequences. We conducted molecular dynamics simulations of Asp and Glu peptides in the presence of calcium and chloride ions to elucidate the underlying phenomena. In line with experimental differences, in our simulations, we indeed find strong differences in the way the peptides interact with ions in solution. The investigated Asp pentapeptide tends to pull a lot of ions into its vicinity, and many structures with clusters of calcium and chloride ions on the surface of the peptide can be observed. Under the same conditions, comparatively fewer ions can be found in proximity of the investigated Glu pentapeptide, and the structures are characterized by single calcium ions bound to multiple carboxylate groups. Based on our simulation data, we identified three reasons contributing to these differences, leading to a new level of understanding additive-ion interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspartic Acid*
  • Calcium
  • Glutamic Acid*
  • Ions
  • Peptides

Substances

  • Ions
  • Peptides
  • Aspartic Acid
  • Glutamic Acid
  • Calcium