RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies

Ka-Ning To; Oliver Powell; Dorota Jamrozy; Rachel Kopunova; Kyriaki Anastasiadou; Amadou Faal; Ousman Secka; Victoria Chalker; Kirsty Le Doare; Elita Jauneikaite

doi:10.1016/j.mimet.2021.106322

RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies

J Microbiol Methods. 2021 Nov:190:106322. doi: 10.1016/j.mimet.2021.106322. Epub 2021 Sep 20.

Authors

Affiliations

¹ Department of Infectious Disease, Imperial College London, Norfolk Place, London W2 1PG, UK; Institute of Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: k.to15@imperial.ac.uk.
² MRC Centre for Molecular Bacteriology and Infection, Department of Infectious Disease, Imperial College London, 14 Armstrong Road, London SW7 2DD, UK. Electronic address: o.powell18@imperial.ac.uk.
³ Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK. Electronic address: dj9@sanger.ac.uk.
⁴ Department of Medicine, Imperial College London, Norfolk Place, London W2 1PG, UK. Electronic address: rachel.kopunova17@imperial.ac.uk.
⁵ Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, Norfolk Place, London W2 1PG, UK.
⁶ Microbiology Laboratories, MRC Unit The Gambia at LSHTM, Fajara, P. O. Box 273, The Gambia. Electronic address: afaal@mrc.gm.
⁷ Microbiology Laboratories, MRC Unit The Gambia at LSHTM, Fajara, P. O. Box 273, The Gambia. Electronic address: Ousman.Secka@lshtm.ac.uk.
⁸ Respiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England, 61 Colindale, London NW9 5EQ, UK. Electronic address: Vicki.Chalker@phe.gov.uk.
⁹ Department of Infectious Disease, Imperial College London, Norfolk Place, London W2 1PG, UK; Institute of Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: kiledoar@sgul.ac.uk.
¹⁰ Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, Norfolk Place, London W2 1PG, UK; NHIR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Department of Infectious Disease, Imperial College London, Hammersmith Campus, Commonwealth Building, London, W12 0NN, UK. Electronic address: e.jauneikaite@imperial.ac.uk.

Abstract

Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.

Keywords: Co-colonisation; Genetic diversity; Group B Streptococcus; Multiple serotypes; RAPD PCR; S. agalactiae; Screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial
Female
Humans
Infant
Mass Screening / methods*
Milk, Human / microbiology
Nasopharynx / microbiology
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide
Random Amplified Polymorphic DNA Technique / methods*
Rectum / microbiology
Serogroup
Streptococcal Infections / diagnosis
Streptococcal Infections / microbiology
Streptococcus agalactiae / genetics*
Streptococcus agalactiae / isolation & purification*
Vagina / microbiology
Whole Genome Sequencing

Substances

DNA, Bacterial

Abstract

Publication types

MeSH terms

Substances

Grants and funding