Higher proportion of non-classical and intermediate monocytes in newly diagnosed multiple myeloma patients in Egypt: A possible prognostic marker

Asmaa M Zahran; Hanaa Nafady-Hego; Sawsan M Moeen; Hanan A Eltyb; Mohammed M Wahman; Asmaa Nafady

doi:10.4102/ajlm.v10i1.1296

Higher proportion of non-classical and intermediate monocytes in newly diagnosed multiple myeloma patients in Egypt: A possible prognostic marker

Afr J Lab Med. 2021 Aug 25;10(1):129. doi: 10.4102/ajlm.v10i1.1296. eCollection 2021.

Authors

Asmaa M Zahran¹, Hanaa Nafady-Hego², Sawsan M Moeen³, Hanan A Eltyb⁴, Mohammed M Wahman⁵, Asmaa Nafady⁶

Affiliations

¹ Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
² Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
³ Department of Internal Medicine, Clinical Haematology Unit, Faculty of Medicine, Assiut University, Assiut, Egypt.
⁴ Department of Medical Oncology, South Egypt Cancer Institute, Assiut University Assiut, Egypt.
⁵ Department of Clinical Oncology, South Valley University, Qena, Egypt.
⁶ Department of Clinical and Chemical Pathology, Qena Faculty of Medicine, South Valley University, Qena, Egypt.

Abstract

Background: Interaction between multiple myeloma (MM) cells and proximal monocytes is expected during plasma cell proliferation. However, the role of monocyte subsets in the disease progression is unknown.

Objective: This study evaluated circulating monocyte populations in MM patients and their correlation with disease severity.

Methods: Peripheral monocytes from 20 patients with MM attending Assiut University Hospital in Assiut, Egypt, between October 2018 and August 2019 were processed using a flow cytometry procedure and stratified using the intensity of expression of CD14 and CD16 into classical (CD16^-CD14⁺⁺), intermediate (CD16⁺CD14⁺⁺), and non-classical (CD16⁺⁺CD14⁺) subsets. The data were compared with data from 20 healthy control participants with comparable age and sex.

Results: In patients with MM, the percentage of classical monocytes was significantly lower (mean ± standard error: 77.24 ± 0.66 vs 83.75 ± 0.5), while those of non-classical (12.44 ± 0.5 vs 8.9 ± 0.34) and intermediate (10.3 ± 0.24 vs 7.4 ± 0.29) monocytes were significantly higher when compared with those of controls (all p < 0.0001). Proportions of non-classical and intermediate monocytes correlated positively with serum levels of plasma cells, M-protein, calcium, creatinine and lactate dehydrogenase, and correlated negatively with the serum albumin level. Proportions of classical monocytes correlated positively with albumin level and negatively correlated with serum levels of M-protein, plasma cells, calcium, creatinine, and lactate dehydrogenase.

Conclusion: Circulating monocyte subpopulations are skewed towards non-classical and intermediate monocytes in MM patients, and the intensity of this skewness increases with disease severity.

Keywords: classical monocytes; flow cytometry; intermediate monocytes; multiple myeloma; non-classical monocytes.