The Effect of Oxytocin on Intracellular Ca2+ Release in Cardiac Cells

Methods Mol Biol. 2022:2384:43-52. doi: 10.1007/978-1-0716-1759-5_3.

Abstract

Ca2+ signaling is vital for the proper functioning of all cells, including cells of the cardiovascular system. Membrane receptors for many hormones trigger intracellular Ca2+ signaling via the activation of phospholipase C and production of inositol-1,4,5-trisphosphate (InsP3). Several research groups have demonstrated the expression of oxytocin (OXT) and oxytocin receptors (OXTR) in the heart and suggested a cardioprotective role of OXT against several pathological conditions. Here we describe the protocol for measuring the effects of oxytocin on intracellular Ca2+ dynamics in newborn rat cardiac myocytes and cardiac fibroblasts maintained in short-term culture.

Keywords: Ca2+ signaling; Cal-520; Cardiac fibroblast; Cardiac myocyte; Endoplasmic reticulum; IP3 receptors; Oxytocin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium Signaling*
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Inositol 1,4,5-Trisphosphate Receptors / metabolism
  • Intracellular Space
  • Myocytes, Cardiac* / metabolism
  • Oxytocin
  • Rats

Substances

  • Inositol 1,4,5-Trisphosphate Receptors
  • Oxytocin
  • Inositol 1,4,5-Trisphosphate
  • Calcium