Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling

Methods Mol Biol. 2022:2549:335-344. doi: 10.1007/7651_2021_427.

Abstract

The generation of cardiomyocytes (CMs) and endothelial cells (ECs) from human induced pluripotent stem cells (iPSCs) allows for precise modeling of cardiovascular disease using clinically relevant and patient-specific cells. Differentiation of human iPSCs into cardiomyocytes (iPSC-CMs) and endothelial cells (iPSC-ECs) is governed by small molecules that regulate the WNT signaling pathway. Here we outline the detailed steps to generate iPSC-CMs and iPSC-ECs through small molecule-mediated monolayer differentiation.

Keywords: Cardiomyocytes; Endothelial cells; Human induced pluripotent stem cells (iPSCs); iPSC-CMs; iPSC-ECs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Differentiation
  • Endothelial Cells
  • Humans
  • Induced Pluripotent Stem Cells*
  • Myocytes, Cardiac
  • Wnt Signaling Pathway