Discovery of DS68702229 as a Potent, Orally Available NAMPT (Nicotinamide Phosphoribosyltransferase) Activator

Chem Pharm Bull (Tokyo). 2021;69(11):1110-1122. doi: 10.1248/cpb.c21-00700.

Abstract

Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step of the nicotinamide adenine dinucleotide (NAD+) salvage pathway. Because NAD+ plays a pivotal role in energy metabolism and boosting NAD+ has positive effects on metabolic regulation, activation of NAMPT is an attractive therapeutic approach for the treatment of various diseases, including type 2 diabetes and obesity. Herein we report the discovery of 1-(2-phenyl-1,3-benzoxazol-6-yl)-3-(pyridin-4-ylmethyl)urea 12c (DS68702229), which was identified as a potent NAMPT activator. Compound 12c activated NAMPT, increased cellular NAD+ levels, and exhibited an excellent pharmacokinetic profile in mice after oral administration. Oral administration of compound 12c to high-fat diet-induced obese mice decreased body weight. These observations indicate that compound 12c is a promising anti-obesity drug candidate.

Keywords: anti-obesity; benzoxazole; body weight reduction; nicotinamide adenine dinucleotide (NAD+); nicotinamide phosphoribosyltransferase activator.

MeSH terms

  • Animals
  • Anti-Obesity Agents / administration & dosage
  • Anti-Obesity Agents / chemical synthesis*
  • Anti-Obesity Agents / pharmacokinetics
  • Body Weight / drug effects
  • Diabetes Mellitus, Type 2 / metabolism
  • Humans
  • Male
  • Mice
  • Mice, Obese
  • NAD / metabolism
  • Nicotinamide Phosphoribosyltransferase / metabolism*
  • Obesity / metabolism
  • Small Molecule Libraries / administration & dosage
  • Small Molecule Libraries / chemical synthesis*
  • Small Molecule Libraries / pharmacokinetics
  • Structure-Activity Relationship
  • Urea / administration & dosage
  • Urea / chemical synthesis*
  • Urea / pharmacokinetics

Substances

  • Anti-Obesity Agents
  • Small Molecule Libraries
  • NAD
  • Urea
  • Nicotinamide Phosphoribosyltransferase