Sulfidation forwarding high-strength Anammox process using nitrate as electron acceptor via thiosulfate-driven nitrate denitratation

Wenmin Lin; Jiyu Feng; Keqiang Hu; Bingyu Qu; Song Song; Kuang He; Chang Liu; Yuancai Chen; Yongyou Hu

doi:10.1016/j.biortech.2021.126335

Sulfidation forwarding high-strength Anammox process using nitrate as electron acceptor via thiosulfate-driven nitrate denitratation

Bioresour Technol. 2022 Jan;344(Pt B):126335. doi: 10.1016/j.biortech.2021.126335. Epub 2021 Nov 14.

Authors

Wenmin Lin¹, Jiyu Feng¹, Keqiang Hu¹, Bingyu Qu¹, Song Song¹, Kuang He², Chang Liu², Yuancai Chen³, Yongyou Hu¹

Affiliations

¹ Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
² South China Institute of Environmental Sciences, MEE, Guangzhou, 510006, China.
³ Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China. Electronic address: chenyc@scut.edu.cn.

PMID: 34785327
DOI: 10.1016/j.biortech.2021.126335

Abstract

A single up-flow thiosulfate-driven nitrate denitratation coupled with the sulfurized Anammox (TDSA) with the core-shell structure (S⁰@ Anammox granules) provided a chemical/energy-saving way for the removal of high-content ammonium with nitrate as electron acceptor. Approximately 83.66% total nitrogen removal efficiency (TNRE) could be achieved by the sulfurized Anammox encrusted by S⁰/S_n^2- at a high loading rate (2.6 kg-N/(m³·d)) via resisting high concentration of free ammonia (FA) (22.35 mg/L), mainly through S₂O₃^2-, S⁰/S_n^2- -driven partial denitrification-Anammox (PDN-Anammox) process. Moreover, S⁰/S_n^2--PDN-Anammox was largely restricted when intermittently aerated, but still resulting in 74.47% TNRE due to the partial nitrification-Anammox (PN-Anammox). The sequencing analysis revealed that Anammox bacterium (Candidatus_Kuenenia) and sulfur-oxidizing bacterium (Thiobacillus) coexisted, in which Anammox process occurred mainly via NO instead of NH₂OH. This study provided a new perspective for high concentration nitrogen wastewater removal in engineering applications.

Keywords: Anammox; Autotrophic denitrification; High ammonium wastewater; Nitrogen removal; Sulfur-oxidizing bacteria.

MeSH terms

Ammonium Compounds*
Anaerobic Ammonia Oxidation
Bioreactors
Denitrification
Electrons
Nitrates*
Nitrogen / analysis
Oxidation-Reduction
Thiosulfates
Wastewater / analysis

Substances

Ammonium Compounds
Nitrates
Thiosulfates
Waste Water
Nitrogen