Evaluation of SARS-CoV-2 antibody responses in healthcare workers at a tertiary care COVID 19 dedicated hospital in Delhi

Indian J Med Microbiol. 2022 Jan-Mar;40(1):30-34. doi: 10.1016/j.ijmmb.2021.11.006. Epub 2021 Nov 18.

Abstract

Purpose: Amidst the on-going SARS-CoV-2 pandemic, healthcare workers have been at a greater risk of disease exposure as they are working in environments chiefly involved in the COVID-19 patient care since March 2020. SARS-CoV-2 antibody testing can prove to be a valuable tool for better understanding of prevalence of disease exposure in this population. Therefore, we conducted this study to grasp the sero-prevalence of COVID-19 antibodies in our hospital to better comprehend the duration of IgG response.

Methods: This was a longitudinal study involving 305 healthcare workers at Rajiv Gandhi Super Speciality Hospital spanning over a period of four months starting from October 2020 to January 2021. Serum samples were obtained from the study group taken as Day 0 of the study and were screened for the presence of SARS-CoV-2 IgG antibodies using semi-quantitive enzyme linked immunoassay technology from ERBAlisa (India). The Antibody Index was determined. Those showing reactive in the screening test were further followed up on a monthly basis till January 2021 for serial antibody testing.

Results: The overall seroprevalence for IgG response among the workers was found to be 21.96%. Seropositivity rate was observed to be significantly higher in those having a history of RT-PCR confirmed COVID-19 infection (45.09%) CONCLUSIONS: Our study demonstrated that healthcare workers have a higher sero-prevalence. Our study also demonstrated that the antibodies developed following COVID-19 infection had a waning effect of protective response following infection.

Keywords: Antibodies; COVID-19; Immunology; Serology; Virology.

MeSH terms

  • Antibodies, Viral
  • Antibody Formation
  • COVID-19* / diagnosis
  • COVID-19* / epidemiology
  • Health Personnel
  • Hospitals
  • Humans
  • Longitudinal Studies
  • SARS-CoV-2
  • Seroepidemiologic Studies
  • Tertiary Healthcare

Substances

  • Antibodies, Viral