Immunoanalytic investigation of grain proteins antigenic for celiac disease patients in an einkorn collection

Zsófia Birinyi; Dalma Réder; Ádám Diós; Ilma R Korponay-Szabó; Éva Hunyadi-Gulyás; Christakis George Florides; Angéla Juhász; Gyöngyvér Gell

doi:10.1016/j.foodchem.2021.131148

Immunoanalytic investigation of grain proteins antigenic for celiac disease patients in an einkorn collection

Food Chem. 2022 Mar 1:371:131148. doi: 10.1016/j.foodchem.2021.131148. Epub 2021 Sep 16.

Authors

Zsófia Birinyi¹, Dalma Réder¹, Ádám Diós², Ilma R Korponay-Szabó³, Éva Hunyadi-Gulyás⁴, Christakis George Florides⁵, Angéla Juhász⁶, Gyöngyvér Gell⁷

Affiliations

¹ Department of Biological Resources, Agricultural Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Martonvásár, HU 2462, Hungary; Doctoral School of Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary.
² Department of Pediatrics, Faculty of Medicine and Clinical Center, University of Debrecen, Debrecen, HU 4032, Hungary; Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, HU 4032, Debrecen, Hungary.
³ Department of Pediatrics, Faculty of Medicine and Clinical Center, University of Debrecen, Debrecen, HU 4032, Hungary; Coeliac Disease Center, Heim Pál National Paediatric Institute, Budapest, HU 1089, Hungary.
⁴ Laboratory of Proteomics Research, Biological Research Centre (BRC), Eötvös Loránd Research Network (ELKH), Szeged, HU 6726, Hungary.
⁵ Murdoch University, College of Science, Health, Engineering and Education, Perth, WA, Australia.
⁶ Edith Cowan University, School of Science, 270 Joondalup Drive, 6027 Joondalup, Western Australia. Electronic address: a.juhasz@ecu.edu.au.
⁷ Department of Biological Resources, Agricultural Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Martonvásár, HU 2462, Hungary; Department of Applied Biotechnology and Food Science, Research Group of Cereal Science and Food Quality, Budapest University of Technology and Economics, Budapest, HU 1111, Hungary. Electronic address: gell.gyongyver@atk.hu.

PMID: 34808760
DOI: 10.1016/j.foodchem.2021.131148

Abstract

Our study focuses on the complex characterization of a wild and cultivated einkorn collection of the Cereal Gene Bank of Agriculture Research Institute in Hungary, using proteomics, immune analytics and bioinformatics analyses. In a serological ELISA pre-screen of 208 different Triticum monococcum L. ssp. monococcum and Triticum monococcum L. ssp. aegilopoides genotypes with celiac disease samples high diversity was observed in the immune response. Based on the immune analytic results, four genotypes with significantly reduced immune reactivity were selected for detailed proteomics characterization. Our results confirm the benefits of high-throughput/large-scale pre-screening and the use of a complex examination platform to get relevant information about the genetic diversity of celiac disease-relevant proteins in the analyzed einkorn genotypes. These genotypes cannot be incorporated into the daily diet of celiac patients; however, they may represent candidates - especially in combination with enzymatic treatments - to improve the lifestyle of individuals suffering from other clinical conditions like non-celiac wheat sensitivity.

Keywords: Celiac disease; ELISA; Einkorn; HPLC; LC-MS; Prolamin.

MeSH terms

Celiac Disease* / genetics
Edible Grain
Genotype
Grain Proteins*
Humans

Substances

Grain Proteins