QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification

Tyler Dang; Jinbo Wang; Tavia Rucker; Sohrab Bodaghi; Irene Lavagi-Craddock; Georgios Vidalakis

doi:10.1007/978-1-0716-1464-8_21

QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification

Methods Mol Biol. 2022:2316:243-250. doi: 10.1007/978-1-0716-1464-8_21.

Authors

Tyler Dang¹, Jinbo Wang^{1

2}, Tavia Rucker¹, Sohrab Bodaghi¹, Irene Lavagi-Craddock¹, Georgios Vidalakis³

Affiliations

¹ Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
² United States Department of Agriculture-APHIS-Biotechnology Regulatory Service-Biotechnology Risk Assessment Program, Riverdale, MD, USA.
³ Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA. vidalg@ucr.edu.

PMID: 34845700
DOI: 10.1007/978-1-0716-1464-8_21

Abstract

The QuantiGene Plex assay is a molecular non-polymerase chain reaction (PCR)-based multiplex method adapted for citrus viroid detection and identification. Here, we describe the procedures to utilize the QuantiGene Plex assay as a high-throughput screening tool for viroids in purified or crude RNA extracts from citrus tissues.

Keywords: Branched DNA; Branched amplification assay; High-throughput; Luminex MAGPIX®; Multiplex.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Citrus* / genetics
Plant Diseases
Viroids* / genetics