Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation

Qianqian Duan; Jiying Ding; Fangfang Li; Xiaowei Liu; Yunan Zhao; Hongxiu Yu; Yong Liu; Lianjun Zhang

doi:10.3389/fcell.2021.761193

Sirtuin 5 is Dispensable for CD8⁺ T Cell Effector and Memory Differentiation

Front Cell Dev Biol. 2021 Dec 13:9:761193. doi: 10.3389/fcell.2021.761193. eCollection 2021.

Authors

Qianqian Duan^{1

2}, Jiying Ding^{1

2

3}, Fangfang Li^{1

2

4}, Xiaowei Liu^{1

2}, Yunan Zhao⁴, Hongxiu Yu⁵, Yong Liu^{6

7

8}, Lianjun Zhang^{1

2

8}

Affiliations

¹ Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² Suzhou Institute of Systems Medicine, Suzhou, China.
³ School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
⁴ Institute of Biomedical Electromagnetic Engineering, Shenyang University of Technology, Shenyang, China.
⁵ Department of Systems Biology for Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China.
⁶ Cancer Institute, Xuzhou Medical University, Xuzhou, China.
⁷ Center of Clinical Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
⁸ Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, China.

Abstract

CD8⁺ T cell effector and memory differentiation is tightly controlled at multiple levels including transcriptional, metabolic, and epigenetic regulation. Sirtuin 5 (SIRT5) is a protein deacetylase mainly located at mitochondria, but it remains unclear whether SIRT5 plays key roles in regulating CD8⁺ T cell effector or memory formation. Herein, with adoptive transfer of Sirt5^+/+ or Sirt5^-/- OT-1 cells and acute Listeria monocytogenes infection model, we demonstrate that SIRT5 deficiency does not affect CD8⁺ T cell effector function and that SIRT5 is not required for CD8⁺ T cell memory formation. Moreover, the recall response of SIRT5 deficient memory CD8⁺ T cells is comparable with Sirt5^+/+ memory CD8⁺ T cells. Together, these observations suggest that SIRT5 is dispensable for the effector function and memory differentiation of CD8⁺ T cells.

Keywords: CD8 T cell; effector T cell; infecion; memory T cell; sirtuin 5 (SIRT5).