In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Sana Afreen; Amit Rahi; Adriana G Landeros; Manas Chakraborty; Richard J McKenney; Dileep Varma

doi:10.1007/978-1-0716-1904-9_9

In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Methods Mol Biol. 2022:2415:123-138. doi: 10.1007/978-1-0716-1904-9_9.

Authors

Sana Afreen^#¹, Amit Rahi^#¹, Adriana G Landeros¹, Manas Chakraborty¹, Richard J McKenney², Dileep Varma³

Affiliations

¹ Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
² Department of Molecular and Cellular Biology, University of California-Davis, Davis, CA, USA.
³ Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. dileep.varma@northwestern.edu.

^# Contributed equally.

PMID: 34972950
DOI: 10.1007/978-1-0716-1904-9_9

Abstract

The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.

Keywords: Cell cycle; Cell division; Cell synchronization; Chromosome; Kinetochores; Microscopy; Microtubules; Mitosis; Mitotic spindle; Photoactivation; TIR-FM; siRNA.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Chromosome Segregation
Kinetochores*
Microtubules*
Mitosis
Spindle Apparatus

Grants and funding

R01 GM135391/GM/NIGMS NIH HHS/United States