Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality

Wenting Xu; Huajin Li; Liangyue Peng; Liyu Pu; Sijia Xiang; Yue Li; Leiting Tao; Wenbin Liu; Jinhui Liu; Yamei Xiao; Shaojun Liu

doi:10.3389/fcell.2021.817779

Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality

Front Cell Dev Biol. 2022 Jan 20:9:817779. doi: 10.3389/fcell.2021.817779. eCollection 2021.

Authors

Wenting Xu^{1

2}, Huajin Li^{1

2}, Liangyue Peng^{1

2}, Liyu Pu^{1

2}, Sijia Xiang^{1

2}, Yue Li^{1

2}, Leiting Tao^{1

2}, Wenbin Liu^{1

2}, Jinhui Liu^{1

2}, Yamei Xiao^{1

2}, Shaojun Liu^{1

2}

Affiliations

¹ State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.
² College of Life Sciences, Hunan Normal University, Changsha, China.

Abstract

The technique of induced pluripotent stem cells has significant application value in breeding and preserving the genetic integrity of fish species. However, it is still unclear whether the chemically induced pluripotent stem cells can be induced from non-mammalian cells or not. In this article, we first verify that fibroblasts of fish can be chemically reprogrammed into pluripotent stem cells. These induced pluripotent stem-like cells possess features of colony morphology, expression of pluripotent marker genes, formation of embryoid bodies, teratoma formation, and the potential to differentiate into germ cell-like cells in vitro. Our findings will offer a new way to generate induced pluripotent stem cells in teleost fish and a unique opportunity to breed commercial fish and even save endangered fish species.

Keywords: chemical reprogramming; fish; germ cell; induced pluripotent stem cell; small molecule compounds.