Blue light induces ROS mediated apoptosis and degradation of AML1-ETO oncoprotein in Kasumi-1 cells

Med Oncol. 2022 Feb 12;39(5):52. doi: 10.1007/s12032-022-01650-x.

Abstract

Light-emitting diode (LED)-based therapies, particularly blue LEDs with wavelengths of 400-500 nm, have shown beneficial results in several cancers, including melanoma, lymphoid cells, and skin tumors. In this study, the cell viability and apoptosis of Kasumi-1 cells treated by blue light (BL) irradiation have been explored. Firstly, BL can specially inhibit the proliferation and promote the apoptosis of Kasumi-1 cells. Furthermore, the apoptosis was triggered by the production of reactive oxygen species and the decline of mitochondrial membrane potential which was regulated by the ratio of Bcl-2(Bcl-xL)/Bax; BL caused the cells' final apoptosis accompanied with the increased cleavage of caspase-3 and poly-ADP-ribose polymerase. Finally, BL induced the degradation of AML1-ETO dependent on the activation of caspase-3. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of Kasumi-1 cells.

Keywords: AML1-ETO; Apoptosis; Caspase-3; Cell viability; Kasumi-1.

MeSH terms

  • Apoptosis / radiation effects*
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • Cell Survival / radiation effects*
  • Color
  • Core Binding Factor Alpha 2 Subunit / metabolism*
  • Core Binding Factor Alpha 2 Subunit / radiation effects
  • Humans
  • Membrane Potential, Mitochondrial / radiation effects*
  • Oncogene Proteins, Fusion / metabolism*
  • Oncogene Proteins, Fusion / radiation effects
  • Photic Stimulation / methods
  • Poly(ADP-ribose) Polymerases / metabolism
  • RUNX1 Translocation Partner 1 Protein / metabolism*
  • RUNX1 Translocation Partner 1 Protein / radiation effects
  • Reactive Oxygen Species / radiation effects*

Substances

  • AML1-ETO fusion protein, human
  • Core Binding Factor Alpha 2 Subunit
  • Oncogene Proteins, Fusion
  • RUNX1 Translocation Partner 1 Protein
  • Reactive Oxygen Species
  • Poly(ADP-ribose) Polymerases
  • Caspase 3