Immunohistological reactivity pattern of the anti-cutaneous T-cell lymphoma antibody BE2

Br J Dermatol. 1986 Jun;114(6):677-84. doi: 10.1111/j.1365-2133.1986.tb04875.x.

Abstract

It has been reported that the monoclonal antibody BE2, raised against leukaemic T cells, reacts specifically with malignant lymphoid cells and represents a valuable reagent for the early identification of cutaneous T-cell lymphomas. To test this hypothesis, a comprehensive range of nodal and cutaneous biopsies have been examined immunohistologically using single and double immunoenzymatic and immunofluorescent staining methods. BE2 showed a broad range of reactivity, consistently labelling normal endothelial cells, B-lymphocytes in mantle and marginal zones, T-lymphocytes in the paracortex of lymph nodes and medulla of thymus, as well as a variety of different macrophage types, including Langerhans cells and dermal macrophages. Furthermore, although BE2-positive T-lymphocytes were most frequent in malignant lymphomas, they were also found in four of 19 benign dermatoses. We conclude that BE2 is neither T-cell nor tumour-cell specific, and that use of this reagent on tissue sections is unlikely to improve the diagnosis of cutaneous lymphomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Antibodies, Neoplasm / immunology*
  • Antibody Specificity
  • Fluorescent Antibody Technique
  • Humans
  • Immunoenzyme Techniques
  • Lymphoid Tissue / immunology
  • Lymphoma / diagnosis*
  • Lymphoma / immunology
  • Skin / immunology
  • Skin Neoplasms / diagnosis*
  • Skin Neoplasms / immunology
  • T-Lymphocytes / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neoplasm