Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

Mette Richner; Nádia Pereira Gonçalves; Poul Henning Jensen; Jens Randel Nyengaard; Christian Bjerggaard Vægter; Asad Jan

doi:10.1016/j.xpro.2022.101181

Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

STAR Protoc. 2022 Feb 17;3(1):101181. doi: 10.1016/j.xpro.2022.101181. eCollection 2022 Mar 18.

Authors

Mette Richner¹, Nádia Pereira Gonçalves¹, Poul Henning Jensen², Jens Randel Nyengaard³, Christian Bjerggaard Vægter¹, Asad Jan²

Affiliations

¹ Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic-EMBL Partnership for Molecular Medicine, Aarhus University, Høegh-Guldbergs Gade 10, 8000 Aarhus C, Denmark.
² DANDRITE, Department of Biomedicine, Aarhus University, Ole Worms Allé 3, 8000 Aarhus C, Denmark.
³ Core Center for Molecular Morphology, Section for Stereology and Microscopy Department of Clinical Medicine, Aarhus University, Department of Pathology, Aarhus University Hospital, 8200 Aarhus N, Denmark.

Abstract

This protocol outlines a minimally invasive and quickly performed approach for transgene delivery in the extracranial nervous system of adult mice using recombinant adeno-associated virus (AAV). The technique, named Sciatic Nerve Direct Immersion (SciNDi), relies on the direct bilateral immersion of the exposed sciatic nerve with AAV. We show that in comparison with intramuscular AAV delivery, SciNDi results in widespread transduction in connected neuroanatomical tracts both in the sciatic nerve trunk and the lumbar spinal cord. For complete details on the use and execution of this protocol, please refer to Jan et al. (2019) and Richner et al. (2011, 2017).

Keywords: Gene Expression; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dependovirus* / genetics
Genetic Vectors / genetics
Immersion*
Mice
Spinal Cord
Transduction, Genetic