Generation of highly pure motor neurons from human induced pluripotent stem cells

Masuma Akter; Haochen Cui; Masood Sepehrimanesh; Md Abir Hosain; Baojin Ding

doi:10.1016/j.xpro.2022.101223

Generation of highly pure motor neurons from human induced pluripotent stem cells

STAR Protoc. 2022 Mar 10;3(1):101223. doi: 10.1016/j.xpro.2022.101223. eCollection 2022 Mar 18.

Authors

Masuma Akter^{1

2}, Haochen Cui¹, Masood Sepehrimanesh¹, Md Abir Hosain¹, Baojin Ding¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
² Department of Biology, University of Louisiana at Lafayette, 410 East Saint Mary Boulevard, Lafayette, LA 70503, USA.

Abstract

Generation of human motor neurons (MNs) overcomes the inaccessibility to patient brain tissues and greatly facilitates the research in MN-related diseases. Here, we describe a protocol for generation of neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs), followed by preparation of functional MNs. The optimized induction condition with the expression of three transcription factors in a single lentiviral vector significantly improved the yield and purity, making it possible to biochemically identify dysregulated factors in diseased neurons. For complete details on the use and execution of this protocol, please refer to Ding (2021), Ding et al. (2021), and Sepehrimanesh and Ding (2020).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Molecular Biology; Neuroscience; Stem Cells.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Differentiation / genetics
Humans
Induced Pluripotent Stem Cells*
Motor Neurons
Neural Stem Cells*
Transcription Factors / genetics

Substances

Transcription Factors

Grants and funding

R21 NS112910/NS/NINDS NIH HHS/United States