Evaluation of commercial ELISA kits for diagnosis of brucellosis in cattle and buffaloes in different epidemiological scenarios

J Microbiol Methods. 2022 Apr:195:106449. doi: 10.1016/j.mimet.2022.106449. Epub 2022 Mar 19.

Abstract

Seven ELISA kits were evaluated for the fitness of purpose in diagnosing brucellosis among cattle and buffaloes in the endemic scenarios of India. The sera (675 numbers) for the study were sourced from brucellosis-free as well as infected herds. The diagnostic sensitivity (dsn) and specificity (dsp) of the kits were determined by three approaches: based on the results of the Rose Bengal test, history of the animals (sera from infected or naïve animals), and based on the results obtained from the 'majority of the tests'. The dsn and dsp ranged from 65.10% to 98.66%, and 98.04% to 100% respectively. The results and suitability of the kits for diagnostic application in various epidemiological situations were discussed.

Keywords: Bovine; Brucellosis; Diagnostic tests; ELISA; Sensitivity; Serology; Specificity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brucellosis* / diagnosis
  • Brucellosis* / epidemiology
  • Brucellosis* / veterinary
  • Buffaloes*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • India / epidemiology
  • Rose Bengal
  • Sensitivity and Specificity

Substances

  • Rose Bengal