An optimized platform for efficient siRNA delivery into human NK cells

Daniel Palacios; Pouria Momayyezi; Oisín Huhn; Eivind Heggernes Ask; Josefine Dunst; Karl-Johan Malmberg; Quirin Hammer

doi:10.1002/eji.202149710

An optimized platform for efficient siRNA delivery into human NK cells

Eur J Immunol. 2022 Jul;52(7):1190-1193. doi: 10.1002/eji.202149710. Epub 2022 Apr 22.

Authors

Daniel Palacios^{1

2}, Pouria Momayyezi³, Oisín Huhn³, Eivind Heggernes Ask^{1

2}, Josefine Dunst^{4

5}, Karl-Johan Malmberg^{1

2}, Quirin Hammer³

Affiliations

¹ Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.
² Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
³ Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden.
⁴ Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden.
⁵ Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden.

Abstract

The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents a promising approach for rapidly interrogating gene functions in primary human NK cells.

Keywords: Gene regulation; Molecular biology; NK cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Killer Cells, Natural*
RNA, Small Interfering / genetics

Substances

RNA, Small Interfering