Application of EPR and related methods to molybdenum-containing enzymes

Russ Hille; Dimitri Niks

doi:10.1016/bs.mie.2022.02.006

Application of EPR and related methods to molybdenum-containing enzymes

Methods Enzymol. 2022:666:373-412. doi: 10.1016/bs.mie.2022.02.006. Epub 2022 Mar 7.

Authors

Russ Hille¹, Dimitri Niks²

Affiliations

¹ Department of Biochemistry, University of California, Riverside, CA, United States. Electronic address: russ.hille@ucr.edu.
² Department of Biochemistry, University of California, Riverside, CA, United States.

PMID: 35465925
DOI: 10.1016/bs.mie.2022.02.006

Abstract

A description is provided of the contributions made to our understanding of molybdenum-containing enzymes through the application of electron paramagnetic resonance spectroscopy and related methods, by way of illustrating how these can be applied to better understand enzyme structure and function. An emphasis is placed on the use of EPR to identify both the coordination environment of the molybdenum coordination sphere as well as the structures of paramagnetic intermediates observed transiently in the course of reaction that have led to the elucidation of reaction mechanism.

Keywords: DMSO reductase; Formate dehydrogenase; Molybdenum enzymes; Nitrate reductase; Sulfite oxidase; Xanthine oxidase.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Electron Spin Resonance Spectroscopy
Molybdenum*

Substances

Molybdenum