Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy

Masato Yamamoto; Satoru Arata; Kunihiko Fukuchi; Hidehiko Honda; Hirokazu Kobayashi; Masahiro Inagaki

doi:10.1016/j.mimet.2022.106474

Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy

J Microbiol Methods. 2022 May:196:106474. doi: 10.1016/j.mimet.2022.106474. Epub 2022 Apr 25.

Authors

Masato Yamamoto¹, Satoru Arata², Kunihiko Fukuchi³, Hidehiko Honda⁴, Hirokazu Kobayashi⁴, Masahiro Inagaki⁴

Affiliations

¹ Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan. Electronic address: yama@cas.showa-u.ac.jp.
² Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan; Center for Biotechnology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
³ Graduate School of Health Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
⁴ Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan.

PMID: 35472329
DOI: 10.1016/j.mimet.2022.106474

Abstract

Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples were also obtained. The infrared spectra of a variety of gasses were measured at high resolution (0.5 cm^-1) to obtain information about the infrared absorption bands to be used as indicators. These key bands enable bacterial classification, and the discrimination rates can be improved by observing multiple infrared absorptions. The air around Pseudomonas aeruginosa was distinguished from the other gas samples by the infrared absorptions at wavenumbers of 778.4 cm^-1 and 2213.2 cm^-1. For Acinetobacter baumannii, infrared absorptions at 1215.0 cm^-1 and 2982.3 cm^-1 were used; furthermore, adding those at 4768.7 cm^-1 and 5353.8 cm^-1 was shown to improve identification.

Keywords: Acinetobacter baumannii; Fourier transform infrared spectroscopy; Infrared absorption; Infrared spectroscopy; Pseudomonas aeruginosa; Volatile metabolite; Volatile organic compound.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii*
Anti-Bacterial Agents / therapeutic use
Gases
Humans
Microbial Sensitivity Tests
Pseudomonas Infections* / microbiology
Pseudomonas aeruginosa
Spectrophotometry, Infrared

Substances

Anti-Bacterial Agents
Gases