Crystal structure of a DNA duplex cross-linked by 6-thioguanine-6-thioguanine disulfides: reversible formation and cleavage catalyzed by Cu(ii) ions and glutathione

Akira Ono; Takahiro Atsugi; Misato Goto; Hisao Saneyoshi; Takahito Tomori; Kohji Seio; Takenori Dairaku; Jiro Kondo

doi:10.1039/c9ra03515j

Crystal structure of a DNA duplex cross-linked by 6-thioguanine-6-thioguanine disulfides: reversible formation and cleavage catalyzed by Cu(ii) ions and glutathione

RSC Adv. 2019 Jul 24;9(40):22859-22862. doi: 10.1039/c9ra03515j. eCollection 2019 Jul 23.

Authors

Akira Ono¹, Takahiro Atsugi¹, Misato Goto¹, Hisao Saneyoshi¹, Takahito Tomori², Kohji Seio², Takenori Dairaku³, Jiro Kondo⁴

Affiliations

¹ Department of Materials & Life Chemistry, Faculty of Engineering, Kanagawa University 3-27-1 Rokkakubashi, Kanagawa-ku Yokohama 221-866 Kanagawa Japan akiraono@kanagawa-u.ac.jp.
² School of Life Science and Technology, Tokyo Institute of Technology 4259 Nagatsuta, Midori-ku Yokohama 226-8501 Japan kseio@bio.titech.ac.jp.
³ School of Pharmaceutical Sciences, Ohu University 31-1 Misumido, Tomita-machi Koriyama Fukushima 963-861 Japan t-dairaku@pha.ohu-u.ac.jp.
⁴ Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University 7-1 Kioi-cho, Chiyoda-ku Tokyo 102-8554 Japan j.kondo@sophia.ac.jp.

Abstract

Herein, we determined the crystal structure of a DNA duplex containing consecutive 6-thioguanine-6-thioguanine disulfides. The disulfide bonds were reversibly formed and cleaved in the presence of Cu(ii) ions and glutathione. To our knowledge, this is the first reaction in which metal ions efficiently accelerated disulfide bond formation between thio-bases in duplexes.