Dendrocalamus latiflorus and its component rutin exhibit glucose-lowering activities by inhibiting hepatic glucose production via AKT activation

Kun Luo; Wenting Huang; Liansheng Qiao; Xiaoling Zhang; Di Yan; Zhiyu Ning; Chengmei Ma; Honglei Dang; Dong Wang; Hongyan Guo; Lan Xie; Jing Cheng

doi:10.1016/j.apsb.2021.11.017

Dendrocalamus latiflorus and its component rutin exhibit glucose-lowering activities by inhibiting hepatic glucose production via AKT activation

Acta Pharm Sin B. 2022 May;12(5):2239-2251. doi: 10.1016/j.apsb.2021.11.017. Epub 2021 Nov 18.

Authors

Kun Luo^{1

2}, Wenting Huang^{1

2}, Liansheng Qiao^{1

2}, Xiaoling Zhang³, Di Yan⁴, Zhiyu Ning^{1

2}, Chengmei Ma³, Honglei Dang³, Dong Wang⁴, Hongyan Guo³, Lan Xie^{1

2

3}, Jing Cheng^{1

2

3

4}

Affiliations

¹ State Key Laboratory of Membrane Biology, School of Medicine, Tsinghua University, Beijing 100084, China.
² Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.
³ National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China.
⁴ School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Abstract

The potential medicinal value of Ma bamboo (Dendrocalamus latiflorus), one of the most popular and economically important bamboo species in China, has been underestimated. In the present study, we found that D. latiflorus leaf extract (DLE) reduced fasting blood glucose levels, body weight, and low-density lipoprotein cholesterol with low liver toxicity in db/db mice. In addition, gene expression profiling was performed and pathway enrichment analysis showed that DLE affected metabolic pathways. Importantly, DLE activated the AKT signaling pathway and reduced glucose production by downregulating glucose-6-phosphatase (G6PC) and phosphoenolpyruvate carboxykinase 1 (PCK1) expression. Moreover, network pharmacology analysis identified rutin as an active component in DLE through targeting insulin growth factor 1 receptor (IGF1R), an upstream signaling transducer of AKT. Due to its hypoglycemic effects and low toxicity, DLE may be considered an adjuvant treatment option for type 2 diabetes patients.

Keywords: AKT; DLE, Dendrocalamus latiflorus leaf extract; Dendrocalamus latiflorus leaf extract; FOXO1; FOXO1, forkhead box O1; G6PC, glucose 6 phosphatase; Gene expression profiling; IGF1R; IGF1R, insulin growth factor 1 receptor; Network pharmacology; PCK1, phosphoenolpyruvate carboxykinase 1; PIN, protein interaction network; PKB/AKT, protein kinase B; Rutin; T2DM, type 2 diabetes mellitus; Type 2 diabetes mellitus.