Selective isolation of hyaluronan by solid phase adsorption to silica

Rebecca MacLeod; Fok Vun Chan; Han Yuan; Xin Ye; Yun Jin Ashley Sin; Teraesa M Vitelli; Tudor Cucu; Annie Leung; Irene Baljak; Samantha Osinski; Yuhong Fu; Gyu Ik Daniel Jung; Anant Amar; Paul L DeAngelis; Urban Hellman; Mary K Cowman

doi:10.1016/j.ab.2022.114769

Selective isolation of hyaluronan by solid phase adsorption to silica

Anal Biochem. 2022 Sep 1:652:114769. doi: 10.1016/j.ab.2022.114769. Epub 2022 Jun 2.

Authors

Rebecca MacLeod¹, Fok Vun Chan², Han Yuan³, Xin Ye⁴, Yun Jin Ashley Sin⁵, Teraesa M Vitelli⁶, Tudor Cucu⁷, Annie Leung⁸, Irene Baljak⁹, Samantha Osinski¹⁰, Yuhong Fu¹¹, Gyu Ik Daniel Jung¹², Anant Amar¹³, Paul L DeAngelis¹⁴, Urban Hellman¹⁵, Mary K Cowman¹⁶

Affiliations

¹ Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: rmacleod@maceps.com.
² Echelon Biosciences Inc., 675 Arapeen Drive, Suite 302, Salt Lake City, UT, 84108, USA. Electronic address: schan@echelon-inc.com.
³ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: julianypoly@gmail.com.
⁴ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: yexin2011@gmail.com.
⁵ Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: yjs287@nyu.edu.
⁶ Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: tmv7271@nyu.edu.
⁷ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: coross2@gmail.com.
⁸ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: al4631@nyu.edu.
⁹ Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: irene96b@gmail.com.
¹⁰ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: samanthaosinski@gmail.com.
¹¹ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: fyhong313@outlook.com.
¹² Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: gj547@nyu.edu.
¹³ Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: anant.amar@my.rfums.org.
¹⁴ Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd., Oklahoma, OK, 73104, USA. Electronic address: paul-deangelis@ouhsc.edu.
¹⁵ Department of Public Health and Clinical Medicine, Umeå University, SE-901 87, Umeå, Sweden. Electronic address: urban.hellman@umu.se.
¹⁶ Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA; Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: mary.cowman@nyu.edu.

Abstract

A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

Keywords: Conditioned medium; Glycosaminoglycan; Hyaluronan; Isolation; Serum; Silica.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Adsorption
Cells, Cultured
Glycosaminoglycans
Humans
Hyaluronic Acid*
Silicon Dioxide*

Substances

Glycosaminoglycans
Silicon Dioxide
Hyaluronic Acid

Grants and funding

R43 GM131444/GM/NIGMS NIH HHS/United States