Reprogramming TracrRNAs for Multiplexed RNA Detection

Chunlei Jiao; Chase L Beisel

doi:10.1007/978-1-0716-2421-0_13

Reprogramming TracrRNAs for Multiplexed RNA Detection

Methods Mol Biol. 2022:2518:217-235. doi: 10.1007/978-1-0716-2421-0_13.

Authors

Chunlei Jiao¹, Chase L Beisel^{2

3}

Affiliations

¹ Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), Würzburg, Germany.
² Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), Würzburg, Germany. Chase.Beisel@helmholtz-hiri.de.
³ Medical Faculty, University of Würzburg, Würzburg, Germany. Chase.Beisel@helmholtz-hiri.de.

PMID: 35666448
DOI: 10.1007/978-1-0716-2421-0_13

Abstract

CRISPR-based detection and recording technologies are gaining increasing attention in disease surveillance and prevention. In this chapter, we describe how our recent discovery of noncanonical crRNAs inspired the engineering of reprogrammed tracrRNAs and led to a powerful platform for multiplexed RNA detection. We provide detailed protocols regarding how to design reprogrammed tracrRNA and carry out assays in vitro and in vivo.

Keywords: CRISPR; Noncanonical crRNA; RNA detection; Reprogrammed tracrRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
RNA* / genetics
RNA, Guide, CRISPR-Cas Systems* / genetics

Substances

RNA, Guide, CRISPR-Cas Systems
RNA