Hymenobacter cyanobacteriorum sp. nov., isolated from a freshwater reservoir during the cyanobacterial bloom period

Abstract

A Gram-negative, red-colored, and rod-shaped bacterial strain, DH14^T, was isolated from a eutrophic reservoir. The 16S rRNA gene sequence analysis showed that strain DH14^T was most closely related to Hymenobacter terrigena (98.3% similarity) and Hymenobacter terrae (98.1%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DH14^T and its related type strains were below 82.9% and 27.2%, respectively. Strain DH14^T contained iso-C_15:0 (32.6%), anteiso-C_15:0 (14.0%), and summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c) (25.8%) as major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, and one unidentified lipid. The respiratory quinone was menaquinone 7 (MK-7). The genomic DNA G + C content was 62.1%. These evidences support the classification of strain DH14^T as a novel species in the genus Hymenobacter, for which the name Hymenobacter cyanobacteriorum sp. nov. is proposed. The type strain is DH14^T (= KCTC 92040^T = LMG 32425^T).

Keywords: 16S rRNA gene; Cyanobacterial bloom; Hymenobacter; Polyphasic taxonomy.