N addition rebalances the carbon and nitrogen metabolisms of Leymus chinensis through leaf N investment

Abstract

Intensifying nitrogen (N) deposition disturbs the growth of grassland plants due to an imbalance between their carbon (C) and N metabolism. However, it's unclear how plant physiological strategies restore balance. We investigated the effects of multiple N addition levels (0-25 g N m^-2 yr^-1) on the coordination of C and N metabolism in a dominant grass (Leymus chinensis) in a semiarid grassland in northern China. To do so, we evaluated photosynthetic parameters, leaf N allocation, C- and N-based metabolites, and metabolic enzymes. We found that a moderate N level (10 g N m^-2 yr^-1) promoted carboxylation and electron transport by allocating more N to the photosynthetic apparatus and increasing ribulose bisphosphate carboxylase/oxygenase activity, thereby increasing photosynthetic capacity. The highest N level (25 g N m^-2 yr^-1) promoted N investment in nonphotosynthetic pathways and increased the free amino acids in the leaves. N addition stimulated the accumulation of C and N compounds across organs by activating sucrose phosphate synthase, nitrate reductase, and glutamine synthetase. This enhancement triggered a transformation of primary metabolites (nonstructural carbohydrates, proteins, amino acids) to secondary metabolites (flavonoids, phenols, and alkaloids) for temporary storage or as defense compounds. Citric acid, as the C skeleton for enhanced N metabolism, decreased significantly, and malic acid increased by catalysis of phosphoenolpyruvate carboxylase. Our findings show the adaptability of L. chinensis to different N-addition levels by adjusting its allocations of C and N metabolic compounds and confirm the roles of C and N coordination by grassland plants in these adaptations.

Keywords: C metabolism; Leaf N allocation; Leymus chinensis; N metabolism; Nitrogen addition.