Anti-inflammatory effects of N-Acetylcysteine and Elaeagnus angustifolia extract on acute lung injury induced by λ-carrageenan in rat

Inflammopharmacology. 2022 Oct;30(5):1759-1768. doi: 10.1007/s10787-022-01003-0. Epub 2022 Jun 20.

Abstract

N-Acetylcysteine (NAC) is a chemical compound with anti-inflammatory and antioxidant activity and acts as a free radical scavenger. Elaeagnus angustifolia (EA) is a plant native to the western part of Iran, with antioxidant and anti-inflammatory properties. The present study been taken evaluated the protective effect afforded by EA and NAC extracts on carrageenan-induced acute lung injury in Wistar rats. In this study, 42 rats were randomly assigned into seven groups. NAC and EA extracts were orally administered once/day for 21 continuous days. Pulmonary damage was induced by intratracheal injection of 100 μl of 2% λ-Carrageenan on day 21. Twenty-four hours post-surgery, the rats were euthanized and the samples were collected. Pretreatment with NAC and EA extracts reduced the total and differential cell accumulation as well as IL-6, and TNF-α cytokines. Antioxidant indicators demonstrate that in the groups receiving NAC and EA extract, MDA decreased while thiol and antioxidant capacity elevated. Treatment with NAC and EA significantly reduced Carrageenan-induced pathological pulmonary tissue injury. NAC and EA extract has protective effects on acute carrageenan-induced lung injury.

Keywords: Acute lung injury; Anti-inflammatory; Elaeagnus angustifolia; N-acetylcysteine; λ-carrageenan.

MeSH terms

  • Acetylcysteine / pharmacology
  • Acute Lung Injury* / chemically induced
  • Acute Lung Injury* / drug therapy
  • Acute Lung Injury* / pathology
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / pharmacology
  • Carrageenan / pharmacology
  • Cytokines
  • Elaeagnaceae* / chemistry
  • Free Radical Scavengers / pharmacology
  • Interleukin-6
  • Lung
  • Plant Extracts / pharmacology
  • Rats
  • Rats, Wistar
  • Sulfhydryl Compounds / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Cytokines
  • Free Radical Scavengers
  • Interleukin-6
  • Plant Extracts
  • Sulfhydryl Compounds
  • Tumor Necrosis Factor-alpha
  • Carrageenan
  • Acetylcysteine