Emergence of bla OXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Haishan Mo; Xu Lin; Ming Liu; Hui Liang

doi:10.2147/IDR.S363019

Emergence of bla _OXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Infect Drug Resist. 2022 Jun 18:15:3173-3177. doi: 10.2147/IDR.S363019. eCollection 2022.

Authors

Haishan Mo^#¹, Xu Lin^#¹, Ming Liu², Hui Liang¹

Affiliations

¹ Department of General Surgery, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People's Republic of China.
² College of Oceanography, Hohai University, Nanjing, People's Republic of China.

^# Contributed equally.

Abstract

Cephalosporins and azithromycin are clinical antibiotics used to treat infections. Co-resistance to cephalosporins and azithromycin has been observed in some Enterobacterales, but it has only rarely been reported in Vibrio species. In this study, we isolated a cephalosporin- and azithromycin-resistant V. furnissii strain, VFN3, from hospital sewage. Whole-genome sequencing results showed that the strain VFN3 possesses an IncA/C2 plasmid, pVFN3-blaOXA-193K. This conjugative plasmid carries several clinically relevant drug resistance genes, including mph(A) and bla _OXA-1. We also found that in the strain VFN3, mph(A) and bla _OXA-1 are surrounded by insertion sequences and class I integrons, respectively. These data suggest that mobile elements mediate the transfer of mph(A) and bla _OXA-1. This is the first reported Vibrio species that possesses an mph(A)- and bla _OXA-1-bearing conjugative plasmid. The emergence of this conjugative multi-drug-resistance plasmid is of great concern to public health.

Keywords: Vibrio furnissii; blaOXA-1; conjugative plasmid; mph(A).