Protocol for assessing ex vivo lipolysis of murine adipose tissue

Debasish Roy; Julia M Myers; Andrea Tedeschi

doi:10.1016/j.xpro.2022.101518

Protocol for assessing ex vivo lipolysis of murine adipose tissue

STAR Protoc. 2022 Sep 16;3(3):101518. doi: 10.1016/j.xpro.2022.101518. Epub 2022 Jul 1.

Authors

Debasish Roy¹, Julia M Myers², Andrea Tedeschi³

Affiliations

¹ Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. Electronic address: debasish.roy@osumc.edu.
² Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA.
³ Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA; Discovery Theme on Chronic Brain Injury, The Ohio State University, Columbus, OH 43210, USA. Electronic address: andrea.tedeschi@osumc.edu.

Abstract

Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease.

Keywords: Cell-based Assays; Metabolism.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Adipose Tissue* / metabolism
Animals
Intra-Abdominal Fat / metabolism
Lipolysis*
Mice
Receptors, Adrenergic, beta / metabolism
Subcutaneous Tissue / metabolism

Substances

Receptors, Adrenergic, beta

Abstract

Publication types

MeSH terms

Substances

Grants and funding