Elevated NF-κB/SHh/GLI1 Signature Denotes a Worse Prognosis and Represent a Novel Potential Therapeutic Target in Advanced Prostate Cancer

Cells. 2022 Jul 5;11(13):2118. doi: 10.3390/cells11132118.

Abstract

Prostate cancer (PCa) is the second most frequent cancer in men worldwide. NF-κB seems to play a key role in cell survival, proliferation and invasion, sustaining the heterogeneous multifocal nature of PCa. In recent years, the Hedgehog (Hh) signaling pathway has attracted attention as a therapeutic target due to its implication in tumorigenesis and metastasis in several types of cancer, including PCa. Although it is well-known that Sonic Hedgehog (SHh) is a transcriptional target of NF-κB(p65), and that GLI1 is the effector of this crosstalk, the precise role played by this axis in PCa is still not completely clear. Here, we set out to explore the correlation between NF-κB activation and SHh pathways in PCa, investigating if the interplay between NF-κB(p65) and SHh-GLI1 in advanced PCa could be a prospective therapeutic target. Our findings demonstrate that a NF-κB-SHh-GLI1 gene signature is enriched in PCa patients featuring a higher Gleason score. Moreover, elevated levels of this signature are associated with worse prognosis, thus suggesting that this axis could provide a route to treat aggressive PCa.

Keywords: GLI1; NF-κB; Sonic Hedgehog; prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Hedgehog Proteins / metabolism
  • Humans
  • Male
  • NF-kappa B* / metabolism
  • Prostatic Neoplasms* / pathology
  • Zinc Finger Protein GLI1 / genetics

Substances

  • GLI1 protein, human
  • Hedgehog Proteins
  • NF-kappa B
  • SHH protein, human
  • Zinc Finger Protein GLI1

Grants and funding

This work was supported by AIRC IG Grant code 5172 and MIUR PRIN Grant code 2009EWAW4M_003 to FZ.