Protocol to establish a stable MLL-AF9 _AML mouse model

Jun Lu; Huanhuan Zhao; Lingling Yang; Xi Jiang

doi:10.1016/j.xpro.2022.101559

Protocol to establish a stable MLL-AF9 _AML mouse model

STAR Protoc. 2022 Jul 18;3(3):101559. doi: 10.1016/j.xpro.2022.101559. eCollection 2022 Sep 16.

Authors

Jun Lu¹, Huanhuan Zhao¹, Lingling Yang¹, Xi Jiang²

Affiliations

¹ Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, Zhejiang 310003, China.
² Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, Zhejiang 310003, China. Electronic address: xjiang@zju.edu.cn.

Abstract

Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse MLL-AF9_AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2022).

Keywords: Cancer; Cell isolation; Cell separation/fractionation; Cell-based Assays.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow Transplantation
Disease Models, Animal
Leukemia, Myeloid, Acute* / genetics
Mice