Codon-specific interaction of uncharged transfer-RNA with eukaryotic ribosomes

Biochim Biophys Acta. 1978 Aug 23;520(1):233-6. doi: 10.1016/0005-2787(78)90024-2.

Abstract

Rat liver ribosomes bound [32P]tRNAPhe in both a codon-dependent and codon-independent manner. The codon-dependent binding was studied further by utilising the ability of the unchanged tRNAPhe to inhibit the poly(U)-directed binding of [3H]Phe-tRNA to ribosomes. At least part of the codon-dependent binding of uncharged tRNA appears to be to the ribosomal A-site.

MeSH terms

  • Binding Sites
  • Codon / metabolism*
  • Escherichia coli
  • Liver
  • Magnesium
  • Phenylalanine
  • Poly U / metabolism
  • RNA, Messenger / metabolism*
  • RNA, Transfer / metabolism*
  • Ribosomes / metabolism*

Substances

  • Codon
  • RNA, Messenger
  • Poly U
  • Phenylalanine
  • RNA, Transfer
  • Magnesium