Assessment of remineralisation potentials of bioactive dental composite using an in-vitro demineralised dentine model

Abstract

Objective: Minimally invasive dentistry encourages conservative caries excavation and remineralisation of the remaining dental tissues. However, dentine remineralisation is more difficult than enamel remineralisation due to the differences in their composition. This study aims to assess the remineralisation potential of Activa BioActive-Restorative and Beautifil II restoration on demineralised dentine samples, and compares it with glass-ionomer (GIC) restoration using energy dispersive X-ray (EDX) and Knoop hardness number (KHN).

Methods: Non-carious extracted molar teeth were used, a total number of ten teeth were sectioned into halves and partially demineralised using 37.0% phosphoric acid for 60 s. All samples are assessed using EDX and KHN prior to restorations. The samples are then subdivided into four groups (n = 5). Group 1 was restored with Activa BioActive-Restorative, Group 2 received Beautifil II, Group 3 was restored with GIC, and the last group was used as a negative control. After storage, the samples were analysed using EDX and KHN.

Results: The demineralisation protocol with 37.0% phosphoric acid significantly decreased the calcium:phosphate (Ca:P) ratio and KHN. Remineralisation occurred in all groups, but the highest percentage change in Ca:P ratio and KHN was observed in the Activa BioActive-Restorative group (20.7%, 82.0%, respectively), followed by the Beautifil II group, glass ionomer group, and the control group, in that order.

Conclusion: Activa BioActive-Restorative restoration presents superior remineralisation compared to Beautifil II and glass-ionomer dental restorations.

Keywords: Activa BioActive-Restorative; Beautifil composite resin; Dentine; Energy dispersive X-Ray spectroscopy; Knoop hardness number.