Microbial electrosynthesis from carbon dioxide feedstock linked to yeast growth for the production of high-value isoprenoids

Abstract

The difficulty in producing multi-carbon and thus high-value chemicals from CO₂ is one of the key challenges of microbial electrosynthesis (MES) and other CO₂ utilization technologies. Here, we demonstrate a two-stage bioproduction approach to produce terpenoids (>C₂₀) and yeast biomass from CO₂ by linking MES and yeast cultivation approaches. In the first stage, CO₂ (C₁) is converted to acetate (C₂) using Clostridium ljungdahlii via MES. The acetate is then directly used as the feedstock to produce sclareol (C₂₀), β-carotene (C₄₀), and yeast biomass using Saccharomyces cerevisiae in the second stage. With the unpurified acetate-containing (1.5 g/L) spent medium from MES reactors, S. cerevisiae produced 0.32 ± 0.04 mg/L β-carotene, 2.54 ± 0.91 mg/L sclareol, and 369.66 ± 41.67 mg/L biomass. The primary economic analysis suggests that sclareol and biomass production is feasible using recombinant S. cerevisiae and non-recombinant S. cerevisiae, respectively, directly from unpurified acetate-containing spent medium of MES.

Keywords: CO(2) fixation; Electricity-driven biosynthesis; Sclareol; Yeast extract; β-carotene.