Corneal imaging with blue-light optical coherence microscopy

Shanjida Khan; Kai Neuhaus; Omkar Thaware; Shuibin Ni; Myeong Jin Ju; Travis Redd; David Huang; Yifan Jian

doi:10.1364/BOE.465707

Corneal imaging with blue-light optical coherence microscopy

Biomed Opt Express. 2022 Aug 30;13(9):5004-5014. doi: 10.1364/BOE.465707. eCollection 2022 Sep 1.

Authors

Shanjida Khan^{1

2}, Kai Neuhaus¹, Omkar Thaware^{1

2}, Shuibin Ni^{1

2}, Myeong Jin Ju^{3

4}, Travis Redd¹, David Huang^{1

2}, Yifan Jian^{1

2

5}

Affiliations

¹ Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA.
² Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR 97239, USA.
³ Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC, Canada.
⁴ School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada.
⁵ jian@ohsu.edu.

Abstract

Corneal imaging is important for the diagnostic and therapeutic evaluation of many eye diseases. Optical coherence tomography (OCT) is extensively used in ocular imaging due to its non-invasive and high-resolution volumetric imaging characteristics. Optical coherence microscopy (OCM) is a technical variation of OCT that can image the cornea with cellular resolution. Here, we demonstrate a blue-light OCM as a low-cost and easily reproducible system to visualize corneal cellular structures such as epithelial cells, endothelial cells, keratocytes, and collagen bundles within stromal lamellae. Our blue-light OCM system achieved an axial resolution of 12 µm in tissue over a 1.2 mm imaging depth, and a lateral resolution of 1.6 µm over a field of view of 750 µm × 750 µm.