Investigating fecal microbial transplant as a novel therapy in dogs with inflammatory bowel disease: A preliminary study

PLoS One. 2022 Oct 18;17(10):e0276295. doi: 10.1371/journal.pone.0276295. eCollection 2022.

Abstract

Background: There are limited studies investigating the use of fecal microbial transplant (FMT) in dogs with inflammatory bowel disease (IBD). The aim of this preliminary study was to assess the feasibility of adding FMT to standard therapy (corticosteroids and a hypoallergenic diet) for dogs with IBD and to and to describe the changes in measured outcomes after 30 days of treatment.

Methods: Thirteen client-owned dogs with IBD were enrolled in this double blinded, randomized clinical trial. All dogs received corticosteroid therapy and a hypoallergenic diet; dogs were randomized to receive either placebo or FMT. Measured outcomes included the canine chronic enteropathy clinical activity index (CCECAI) at 1 week and 1 month after enrolment. Fecal microbiota were analyzed after extracting DNA from fecal samples and profiling using 16S amplicon sequencing. Dogs in the placebo group not responding to treatment after 1 month were offered FMT.

Results: The CCECAI significantly decreased over time in both groups (p = 0.001). There were no significant differences between the CCECAI of the placebo and FMT group at each time point (F test from ANOVA, p = 0.40). No adverse effects were reported in the 30 days following FMT.

Conclusions: The addition of FMT to standard therapy for IBD was feasible. No significant differences were observed in the CCECAI between groups at each time point. Large scale clinical trials can be performed using these methods to evaluate the longer term effect of FMT on clinical signs, microbial diversity, and other outcomes.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dogs
  • Fecal Microbiota Transplantation / methods
  • Feces
  • Inflammatory Bowel Diseases* / etiology
  • Inflammatory Bowel Diseases* / therapy
  • Inflammatory Bowel Diseases* / veterinary
  • Microbiota*
  • Remission Induction
  • Treatment Outcome

Grants and funding

This study was funded by a grant from the Ontario Veterinary College Pet Trust. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. https://pettrust.uoguelph.ca/ Grant # 053377.