Serum pNF-H levels in the first six hours after experimental mild traumatic brain injury in rats

Eur Rev Med Pharmacol Sci. 2022 Oct;26(19):6928-6934. doi: 10.26355/eurrev_202210_29873.

Abstract

Objective: Mild traumatic brain injury (mTBI) cases with a normal CT scanning account for the vast majority of all TBI patients. The aim of this study was to investigate the course of serum Phosphorylated Neurofilament Heavy Chain (pNF-H) levels in the first six hours after trauma in rats in experimental mTBI.

Materials and methods: In this experimental animal study, 32 female Sprague-Dawley rats were enrolled equally (n=8) into 3 experimental groups and 1 control group. In experimental groups, animals were exposed to a mTBI with a free fall of 50-gram metal disc from a height of 80 cm. We compared serum pNF-H levels at the 2nd, 4th, and 6th hours after traumatic brain injury in the experimental groups with the control group.

Results: Serum pNF-H levels at the 2nd and 4th hours after traumatic brain injury were statistically significantly higher than the control group. Serum pNF-H levels gradually decreased at the 4th and 6th hours compared to the 2nd hour and decreased to a similar level to the control group at the 6th hour after injury.

Conclusions: A high serum pNF-H value, could be used in the diagnosis and management of mTBI patients.

MeSH terms

  • Animals
  • Biomarkers
  • Brain Concussion*
  • Brain Injuries, Traumatic* / diagnosis
  • Female
  • Neurofilament Proteins
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Neurofilament Proteins
  • Biomarkers