Analysis of bacterial contamination and the effectiveness of UV light-based reprocessing of everyday medical devices

PLoS One. 2022 Nov 9;17(11):e0268863. doi: 10.1371/journal.pone.0268863. eCollection 2022.

Abstract

Background: The reprocessing of daily used medical devices is often inadequate, making them a potential source of infection. In addition, there are usually no consistent and technically standardized procedures available for this purpose. Hence, the aim of this study is to analyze the bacterial contamination and the effectiveness of Ultraviolet light-based (UV light-based) reprocessing of daily used medical devices.

Material and methods: Six different everyday medical devices (20 each; stethoscopes, tourniquets, bandage scissors, reflex hammers, tuning forks, and nystagmus glasses) were tested for bacterial contamination. All medical devices were then exposed to UV-C light for 25 seconds. Medical devices with a smooth surface were pre-cleaned with a water-based wipe. Contact samples were taken before and after reprocessing.

Results: Immediately after clinical use, 104 of 120 contact samples showed an average bacterial contamination of 44.8±64.3 colony forming units (CFU) (0-300 CFU), also including potentially pathogenic bacteria. Two further culture media were completely overgrown with potentially pathogenic bacteria. The stethoscopes were found to have the highest average contamination of 90±91.6 CFU. After reprocessing, 118 of 120 samples were sterile, resulting in an average residual contamination of 0.02±0.1 CFU in two samples, whereby only bacteria of the ordinary skin flora were found.

Conclusion: The present study shows the potentially clinically relevant bacterial contamination of everyday used medical devices. The reprocessing method tested here using UV light appears to be a suitable method for disinfection, especially for objects that up to now have been difficult to disinfect or cannot be disinfected in a standardized manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria
  • Disinfection / methods
  • Drug Contamination
  • Equipment Contamination* / prevention & control
  • Ultraviolet Rays*

Grants and funding

YES Stefan Alexander Rudhart received personal fees as a consultant and travel grants for congresses from UV Smart Technologies B.V. as a research assistant, outside the submitted work. Boris Alexander Stuck has received research grants for his institution from UV Smart Technologies B.V. UV Smart Technologies B.V. would pay the publication fee in the case of acceptance of the article. As this trail was not funded by a funding programme, it is unfortunately not possible to supply a grant number. Funders website: https://www.uvsmart.nl The sponsors did not play any role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript. All these aspects were elaborated independently of the sponsors by the scientists involved.