Objective: To investigate the relationship of polycyclic aromatic hydrocarbons (PAHs) exposure, S-adenosylhomocysteine hydrolase (SAHH) activity and long noncoding RNA H19 gene expression in the urine of coke oven workers. Methods: In September 2019, in a coking plant in Taiyuan City, 146 male workers who had worked in coke oven operations for one year were selected through a completely random sampling method, and their basic personal information was collected by questionnaire survey, and blood and urine samples were collected. The levels of 4 PAHs metabolites 2-hydroxfluorene (2-FLU), 2- hydroxynaphthalene (2-NAP), 9-hydroxyphenanthren (9-PHE), and 1-hydroxypyrene (1-OHP) in urine were detected by high performance liquid chromatography (HPLC) -fluorescence detection method. HPLC-UV detection method was used to detect the content of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in plasma, and the SAHH activity value was obtained by calculating the ratio. Reverse transcription PCR method was used to determine the H19 gene expression level. Urine levels of 2-FLU, 2-NAP, 9-PHE, and 1-OHP were divided into Q(1), Q(2), Q(3), and Q(4) groups according to quartiles (P(25), P(50), P(75)). Regression, trend test and restricted cubic splines were used to analyze the relationship among PAHs metabolites, SAHH activity, H19 gene expression and their dose-response. Results: The median age of coke oven workers was 39.60 years old, the median length of service was 20.38 years, and the urinary levels of 2-FLU, 2-NAP, 9- PHE, and 1-OHP were 0.29, 0.74, 0.09, and 0.06 μg/mmol Cr, respectively. The levels of 2-FLU, 2-NAP and 9-PHE in the urine of workers were significantly different between groups with different 1-OHP levels (P<0.05). After adjusting for age, length of service, smoking, drinking, and levels of 2-FLU, 2-NAP and 9-PHE, SAHH activity decreased with the increase of urinary 1-OHP level (OR=0.63, 95%CI: 0.41-0.98, P=0.038), showing a nonlinear relationship (P(nonlinear)= 0.030). H19 gene expression increased with the increase of urinary 1- OHP level (OR=1.51, 95%CI: 1.03-2.19, P=0.033), there was a linear relationship (P(trend)= 0.058). The relationship between the other three metabolites in urine and SAHH activity and H19 gene expression was not statistically significant (P>0.05) . Conclusion: Urinary 1-OHP level may be a risk factor for decreased SAHH activity and increased H19 gene expression in coke oven workers.
目的： 探讨焦炉工人尿中多环芳烃（polycyclic aromatic hydrocarbons，PAHs）暴露与S-腺苷高半胱氨酸水解酶（S-adenosylhomocysteine hydrolase，SAHH）活力及长链非编码RNA H19基因表达的关系。 方法： 于2019年9月，在太原市某焦化厂通过完全随机抽样的方法，选取焦炉作业满1年的男性工人146人，问卷调查法收集其个人基本信息，并采集血样和尿样。采用高效液相色谱（high performance liquid chromatography，HPLC）-荧光检测法检测尿中4种PAHs代谢物2-羟基芴、2-羟基萘、9-羟基菲、1-羟基芘的水平；HPLC-紫外检测法检测血浆中S-腺苷蛋氨酸（S-adenosylmethionine，SAM）和S-腺苷高半胱氨酸（S-adenosylhomocysteine，SAH）的含量，通过计算比值得到SAHH活力值；反转录PCR法测定H19基因表达水平。将尿中2-羟基芴、2-羟基萘、9-羟基菲、1-羟基芘水平根据四分位数（P(25)、P(50)、P(75)）各分为Q(1)、Q(2)、Q(3)、Q(4)组，采用多元logistic回归、趋势检验和限制性立方样条分析PAHs代谢物与SAHH活力、H19基因表达的关系及其剂量反应。 结果： 焦炉工人年龄中位数为39.60岁，工龄中位数为20.38年，尿中2-羟基芴、2-羟基萘、9-羟基菲和1-羟基芘水平分别为0.29、0.74、0.09、0.06 μg/mmol Cr。不同1-羟基芘水平组间，工人尿中2-羟基芴、2-羟基萘、9-羟基菲水平差异均有统计学意义（P<0.05）。校正了年龄、工龄、吸烟、饮酒及2-羟基芴、2-羟基萘、9-羟基菲水平后，SAHH活力随尿中1-羟基芘水平的升高而降低（OR=0.63，95%CI：0.41~0.98，P=0.038），表现为非线性关系（P(非线性)=0.030）；H19基因表达水平随尿中1-羟基芘浓度的升高而升高（OR=1.51，95%CI：1.03~2.19，P=0.033），存在线性关系（P(趋势)=0.058）。其他3种尿中代谢物与SAHH活力、H19基因表达的关系均无统计学意义（P>0.05）。 结论： 尿中1-羟基芘水平可能是焦炉工人SAHH活力降低、H19基因表达升高的危险因素。.
Keywords: 1-hydroxypyrene; Coke oven worker; H19; Polycyclic aromatic hydrocarbons; S-adenosylhomocysteine hydrolase; Urine.