Regulation of DC metabolism by nitric oxide in murine GM-CSF cultures

Lucía Minarrieta; Gloria J Godoy; Lis N Velazquez; Peyman Ghorbani; Tim Sparwasser; Luciana Berod

doi:10.1002/eji.202149691

Regulation of DC metabolism by nitric oxide in murine GM-CSF cultures

Eur J Immunol. 2023 Feb;53(2):e2149691. doi: 10.1002/eji.202149691. Epub 2022 Dec 28.

Authors

Lucía Minarrieta¹, Gloria J Godoy², Lis N Velazquez², Peyman Ghorbani¹, Tim Sparwasser^{2

3}, Luciana Berod^{4

3}

Affiliations

¹ Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Canada.
² Institute of Medical Microbiology and Hygiene, University Medical Centre of the Johannes Gutenberg-University Mainz, Mainz, Germany.
³ Research Center for Immunotherapy (FZI), University Medical Center Mainz, Mainz, Germany.
⁴ Institute of Molecular Medicine, University Medical Centre of the Johannes Gutenberg-University Mainz, Mainz, Germany.

PMID: 36577714
DOI: 10.1002/eji.202149691

Abstract

The CD11c⁺ MHCII⁺ compartment within GM-CSF cultures consists of a MHCII^low CD11b^high population (GM-Macs) and a MHCII^high CD11b^int population (GM-DCs), with different metabolic profiles. GM-Macs upregulate iNOS and produce nitric oxide (NO) upon TLR activation inhibiting mitochondrial respiration (OXPHOS) while promoting glycolytic metabolism in GM-DCs, which naturally do not express iNOS.

Keywords: DC metabolism; GM-CSF; dendritic cells; in vitro culture; nitric oxide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Dendritic Cells / metabolism
Granulocyte-Macrophage Colony-Stimulating Factor* / metabolism
Mice
Mice, Inbred C57BL
Nitric Oxide* / metabolism

Substances

Granulocyte-Macrophage Colony-Stimulating Factor
Nitric Oxide