Association between CD34+ and CD3+ T-cells in allogeneic grafts and acute graft-versus-host disease in children undergoing allogeneic hematopoietic stem cell transplantation: A single-center study

Abstract

Background: Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). We examined the association between the composition of the cell subsets present in allogeneic grafts (allografts) and the occurrence and severity of aGVHD in pediatric patients.

Methods: We retrospectively analyzed 80 consecutive pediatric patients undergoing allo-HSCT at our center.

Results: Both univariate and multivariate analyses showed that the number of CD34⁺ and CD3⁺ T-cells in allografts were the two highest risk factors associated with II-IV aGVHD. Using receiver operating characteristic analysis, the cutoff levels of the allo-HSCT cell doses were used to divide the recipients into low-dose and high-dose groups. The 100-day cumulative incidence of II-IV aGVHD in the high-dose CD34⁺ and CD3⁺ T-cells group was significantly higher than that of the low-dose group (CD34⁺: 57% vs. 29%, p = 0.009; CD3⁺: 63% vs. 18%, p < 0.001). No other clinical factors or cell subsets correlated with aGVHD incidence.

Conclusions: Our analysis indicates that the CD34⁺ and CD3⁺ T-cell numbers in the allografts could be the risk factors for the development of severe aGVHD (level II-IV). Further studies should aim to optimize the critical number of CD34⁺ and CD3⁺ T-cells to reduce the risk of severe aGVHD occurrence in pediatric patients.

Keywords: Acute graft-versus-host disease; Allogeneic graft; Allogeneic hematopoietic stem cell transplantation; Cell dose; Children.